Changes in gene expression predictably shift and switch genetic interactions.

ABSTRACT: Non-additive interactions between mutations occur extensively and also change across conditions, making genetic prediction a difficult challenge. To better understand the plasticity of genetic interactions (epistasis), we combine mutations in a single protein performing a single function (a transcriptional repressor inhibiting a target gene). Even in this minimal system, genetic interactions switch from positive (suppressive) to negative (enhancing) as the expression of the gene changes. These seemingly complicated changes can be predicted using a mathematical model that propagates the effects of mutations on protein folding to the cellular phenotype. More generally, changes in gene expression should be expected to alter the effects of mutations and how they interact whenever the relations

SUBMITTER: Li X 

PROVIDER: S-EPMC6715729 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature