A trade-off for covalent and intercalation binding modes: a case study for Copper (II) ions and singly modified DNA nucleoside.

ABSTRACT: Selective binding to nucleic acids and, more generally, to biopolymers, very often requires at a minimum the presence of specific functionalities and precise spatial arrangement. DNA can fold into defined 3D structures upon binding to metal centers and/or lanthanides. Binding efficiency can be boosted by modified nucleosides incorporated into DNA sequences. In this work the high selectivity of modified nucleosides towards copper (II) ions, when used in the monomeric form, is unexpectedly and drastically reduced upon being covalently attached to the DNA sequence in single-site scenario. Surprisingly, such selectivity is partially retained upon non-covalent (i.e. intercalation) mixture formed by native DNA duplex and a nucleoside in the monomeric form. Exploiting the electron spin properties

SUBMITTER: Mouesca JM 

PROVIDER: S-EPMC6715747 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature