Global Analysis of Furfural-Induced Genomic Instability Using a Yeast Model.

ABSTRACT: Furfural is an important renewable precursor for multiple commercial chemicals and fuels; a main inhibitor existing in cellulosic hydrolysate, which is used for bioethanol fermentation; and a potential carcinogen, as well. Using a genetic system in Saccharomyces cerevisiae that allows detection of crossover events, we observed that the frequency of mitotic recombination was elevated by 1.5- to 40-fold when cells were treated with 0.1 g/liter to 20 g/liter furfural. Analysis of the gene conversion tracts associated with crossover events suggested that most furfural-induced recombination resulted from repair of DNA double-strand breaks (DSBs) that occurred in the G₁ phase. Furfural was incapable of breaking DNA directly in vitro but could trigger DSBs in vivo

SUBMITTER: Qi L 

PROVIDER: S-EPMC6715844 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature