Predicting Protein Complex Structure from Surface-Induced Dissociation Mass Spectrometry Data.

ABSTRACT: Recently, mass spectrometry (MS) has become a viable method for elucidation of protein structure. Surface-induced dissociation (SID), colliding multiply charged protein complexes or other ions with a surface, has been paired with native MS to provide useful structural information such as connectivity and topology for many different protein complexes. We recently showed that SID gives information not only on connectivity and topology but also on relative interface strengths. However, SID has not yet been coupled with computational structure prediction methods that could use the sparse information from SID to improve the prediction of quaternary structures, i.e., how protein subunits interact with each other to form complexes. Protein-protein docking, a computational method to predict the qu

SUBMITTER: Seffernick JT 

PROVIDER: S-EPMC6716128 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature