Molecular identification of reovirus in broiler type flocks in Golestan province, Iran.

ABSTRACT:

Background

Avian reovirus (ARV) has a global distribution in nature and most clinical signs are found in broiler type chickens.

Aims

This study was conducted to detect and identify reovirus infections from vaccinated breeder chickens and their progenies.

Methods

A total of 20 tissue and blood samples were collected from vaccinated broiler breeders and their progenies with gastrointestinal or performance problems during peak production. Antibody titers were measured by indirect enzyme-linked immunosorbent assay (ELISA) tests. RNA extraction from tissue samples was performed and cDNA was prepared and directly used in the polymerase chain reaction (PCR). Nucleotide sequences were bilaterally determined using internal primers. The analysis of the nucleotide

SUBMITTER: Mayahi M 

PROVIDER: S-EPMC6716273 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature