Evaluation of immune response to recombinant Bacillus anthracis LFD1-PA4 chimeric protein.

ABSTRACT:

Background

Anthrax is a particularly dangerous infectious disease that affects humans and livestock. Efficacious vaccines that can rapidly induce a long-term immune response are required to prevent anthrax infection in humans. Domains 4 and 1 of the protective antigen (PA) and lethal factor (LF), respectively, have very high antigenic properties.

Aims

In this experimental study, the pET28a-lfD1-pa4 expression vector was designed, constructed and transferred into E. coli BL21 (DE3) plysS.

Methods

For this purpose, pa4 gene was amplified by polymerase chain reaction (PCR) and cloned in a pGEM T-easy vector. The pGEM-pa4 and pGEM-lfD1 were digested by XbaI and HindIII enzymes. The ligation reaction was performed by ligase T4 e

SUBMITTER: Mirhaj H 

PROVIDER: S-EPMC6716276 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature