Metabolomic analysis reveals the mechanism of aluminum cytotoxicity in HT-29 cells.

ABSTRACT:

Background

Aluminum (Al) is toxic to animals and humans. The most common sources of human exposure to Al are food and beverages. The intestinal epithelium is the first barrier against Al-induced toxicity. In this study, HT-29, a human colon cancer cell line, was selected as an in vitro model to evaluate the Al-induced alteration in metabolomic profiles and explore the possible mechanisms of Al toxicity.

Methods

MTT assay was performed to determine the half-maximal inhibitory concentration of Al ions. Liquid chromatography-mass spectrometry (LC-MS) was used for metabolomic analysis, and its results were further confirmed using quantitative reverse transcription polymerase chain reaction (RT-qPCR) of nine selected genes.

Results

Al inhibited the growth of the HT-29 cell

SUBMITTER: Yu L 

PROVIDER: S-EPMC6716502 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature