Light-induced injury in mouse embryos revealed by single-cell RNA sequencing.

ABSTRACT:

Background

Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos.

Results

Here, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and

SUBMITTER: Lv B 

PROVIDER: S-EPMC6716870 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature