CCR6+ group 3 innate lymphoid cells accumulate in inflamed joints in rheumatoid arthritis and produce Th17 cytokines.

ABSTRACT:

Background

Recent studies show that innate lymphoid cells (ILCs) contribute to the development of chronic inflammation and autoimmune disease. In this study, we assessed the ILC function in the development of rheumatoid arthritis (RA).

Methods

In a mouse model of collagen-induced arthritis (CIA), we identified and purified the ILC subsets in peripheral blood (PB), local lymph nodes (LNs), and joints by fluorescence-activated cell sorting and used quantitative PCR to assess the expression levels of representative cytokines. We also correlated the frequencies of each ILC subset in synovial fluid (SF) with clinical parameters in RA patients.

Results

In the CIA model, the proportion of CCR6+ ILC3s to total ILCs in joints with active inflammation significantly increased re

SUBMITTER: Takaki-Kuwahara A 

PROVIDER: S-EPMC6716915 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature