Detecting acid phosphatase enzymatic activity with phenol as a chemical exchange saturation transfer magnetic resonance imaging contrast agent (PhenolCEST MRI).

ABSTRACT: Phenol contains an exchangeable hydroxyl proton resonant at 4.8 ppm from the resonance frequency of water in the ¹H nuclear magnetic resonance (¹H NMR) spectrum, enabling itself to be detected at sub-mM concentration by either chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) or exchange-based T₂ relaxation enhancement (T_2ex) effect under acidic and basic conditions, respectively. We recently investigated the T_2ex effects of phenol and its derivatives, but the CEST characteristics of phenols are unknown in detail, and no study on using the natural CEST MRI effects of phenol for detecting enzymatic activity has been conducted. Herein, on the basis of the inherent CEST MR property of phenol, namely phenolCEST, w

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6717000 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature