Newly developed Laboratory-based Size exclusion chromatography Small-angle x-ray scattering System (La-SSS).

ABSTRACT: To understand a biological system, it is important to observe structures of biomolecules in the solution where the system is functionalized. Small-Angle X-ray Scattering coupled with Size Exclusion Chromatography (SEC-SAXS) is one of techniques to selectively observe the target molecules in the multi-component system. However, this technique is believed to be available only with a synchrotron-based SAXS instrument due to requirement of high beam intensity and, therefore, the limitation of the beam time was obstacle to satisfy demands from many bio-researchers. We newly developed Laboratory-based Size exclusion chromatography SAXS System (La-SSS) by utilizing a latest laboratory-based SAXS instrument and finely optimization of the balance between flow rate, cell volume, irradiation time and

SUBMITTER: Inoue R 

PROVIDER: S-EPMC6717197 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature