De novo transcriptome assembly for four species of crustose coralline algae and analysis of unique orthologous genes.

ABSTRACT: Crustose coralline algae (CCA) are calcifying red macroalgae that reef build in their own right and perform essential ecosystem functions on coral reefs worldwide. Despite their importance, limited genetic information exists for this algal group. De novo transcriptomes were compiled for four species of common tropical CCA using RNA-seq. Sequencing generated between 66 and 87 million raw reads. Transcriptomes were assembled, redundant contigs removed, and remaining contigs were annotated using Trinotate. Protein orthology analysis was conducted between CCA species and two noncalcifying red algae species from NCBI that have published genomes and transcriptomes, and 978 orthologous protein groups were found to be uniquely shared amongst CCA. Functional enrichment analysis of these 'CCA-specif

SUBMITTER: Page TM 

PROVIDER: S-EPMC6717203 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature