Autograft microskin combined with adipose-derived stem cell enhances wound healing in a full-thickness skin defect mouse model.

ABSTRACT:

Objective

Autograft microskin transplantation has been widely used as a skin graft therapy in full-thickness skin defect. However, skin grafting failure can lead to a pathological delay wound healing due to a poor vascularization bed. Considering the active role of adipose-derived stem cell (ADSC) in promoting angiogenesis, we intend to investigate the efficacy of autograft microskin combined with ADSC transplantation for facilitating wound healing in a full-thickness skin defect mouse model.

Material and methods

An in vivo full-thickness skin defect mouse model was used to evaluate the contribution of transplantation microskin and ADSC in wound healing. The angiogenesis was detected by immunohistochemistry staining. In vitro paracrine signaling pathway was evaluated by prot

SUBMITTER: Luo Y 

PROVIDER: S-EPMC6717360 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature