L-Asparaginase production in rotating bed reactor from Rhizopus microsporus IBBL-2 using immobilized Ca-alginate beads.

ABSTRACT: The present work reports on the production of extracellular l-asparaginase from Rhizopus microsporus IBBL-2 using submerged fermentation (SmF) process free of glutaminase and urease activities. Primary studies done in shake flask showed that the highest l-asparaginase activity of 12.68 U mL-1 was produced at 72 h with optimized fermentation parameters such as pH 6.0, 4 × 106 fungal cells mL-1, and agitation of 180 rpm at 30 °C using one-factor-at-a-time (OFAT). Different substrates, nitrogen sources, temperature, pH, the initial number of cells and metal ions were tested to determine the impact on enzyme production. l-Asparaginase activity of 17.68 U mL-1 was produced after 48 h using immobilized calcium-alginate (Ca-alginate) cells. 4 × 106 cells mL-1 was entrapped in 3% (W/V) of alginate

SUBMITTER: Ashok A 

PROVIDER: S-EPMC6717606 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature