The effect of cysteine oxidation on DJ-1 cytoprotective function in human alveolar type II cells.

ABSTRACT: DJ-1 is a multifunctional protein with cytoprotective functions. It is localized in the cytoplasm, nucleus, and mitochondria. The conserved cysteine residue at position 106 (Cys106) within DJ-1 serves as a sensor of redox state and can be oxidized to both the sulfinate (-SO₂^-) and sulfonate (-SO₃^-) forms. DJ-1 with Cys106-SO₂^- has cytoprotective activity but high levels of reactive oxygen species can induce its overoxidation to Cys106-SO₃^-. We found increased oxidative stress in alveolar type II (ATII) cells isolated from emphysema patients as determined by 4-HNE expression. DJ-1 with Cys106-SO₃^- was detected in these cells by mass spectrometry analysis. Moreover, ubiquitination of

SUBMITTER: Bahmed K 

PROVIDER: S-EPMC6717737 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature