Cerium oxide NPs mitigate the amyloid formation of α-synuclein and associated cytotoxicity.

ABSTRACT:

Aim

Among therapeutic proposals for amyloid-associated disorders, special attention has been given to the exploitation of nanoparticles (NPs) as promising agents against aggregation.

Methods

In this paper, the inhibitory effect of cerium oxide (CeO₂) NPs against α-synuclein (α-syn) amyloid formation was explored by different methods such as Thioflavin T (ThT) and 8-anilinonaphthalene-1-sulfonic acid (ANS) fluorescence spectroscopy, Congo red adsorption assay, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), and bioinformatical approaches. Also, the cytotoxicity of α-syn amyloid either alone or with CeO₂ NPs against neuron-like cells (SH-SY5Y) was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MT

SUBMITTER: Zand Z 

PROVIDER: S-EPMC6718255 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature