Development of a multiplex fully automated assay for rapid quantification of CD4⁺ T cells from whole blood.

ABSTRACT: The development of cost-effective and rapid assays for the accurate counting of CD4 cells has remained prime focus for disease management. The lack of such assays has severely affected people living in resource-limited disease prevalent areas. CD4 count information plays a vital role in the effective management of HIV disease. There is an unmet need to develop rapid, cost-effective, portable and user-friendly point-of-care (POC) disease diagnostic platform technology for CD4⁺ T cell counting. Here, we have developed a flow-free magnetic actuation platform that uses antibody-coated magnetic beads to efficiently capture CD4⁺ T cells from a 30 μL drop of whole blood. On-chip cell lysate electrical impedance spectroscopy has been utilized to quantify the isolated CD4 cell

SUBMITTER: Sher M 

PROVIDER: S-EPMC6718319 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature