Multiplex staining depicts the immune infiltrate in colitis-induced colon cancer model.

ABSTRACT: Assessment of the host immune response pattern is of increasing importance as highly prognostic and diagnostic, in immune-related diseases and in some types of cancer. Chronic inflammation is a major hallmark in colon cancer formation, but, despite the extent of local inflammatory infiltrate has been demonstrated to be extremely informative, its evaluation is not routinely assessed due to the complexity and limitations of classical immunohistochemistry (IHC). In the last years, technological advance helped in bypassing technical limits, setting up multiplex IHC (mIHC) based on tyramide signal amplification (TSA) method and designing software suited to aid pathologists in cell scoring analysis. Several studies verified the efficacy of this method, but they were restricted to the analysis of

SUBMITTER: Pivetta E 

PROVIDER: S-EPMC6718623 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature