GATAD1 gene amplification promotes glioma malignancy by directly regulating CCND1 transcription.

ABSTRACT:

Background

The GATAD1 gene overexpression induced by GATAD1 amplification upregulation is detected in different human tumors. To date, the relationship between GATAD1 amplification and glioma oncogenesis and malignancy is still unknown.

Methods

GATAD1 gene amplification and expression were analyzed in 187 gliomas using qPCR and immunostaining. The relation of GATAD1 to patients' prognoses was assessed via the Kaplan-Meier method. The MTT and orthotopic tumor transplantation assays were used to identify the function of GATAD1 in glioma proliferation. cDNA microarray, ChIP qPCR, EMSA and 3C were used to screen the downstream mechanism of GATAD1 regulating glioma proliferation.

Results

Our results indicated that GATAD1 gene amplification and GATAD1 gene expression are no

SUBMITTER: Zhang S 

PROVIDER: S-EPMC6718743 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature