CALIMA: The semi-automated open-source calcium imaging analyzer.

ABSTRACT:

Background and objective

Ever since its discovery, calcium imaging has proven its worth in discovering new insights into the mechanisms of cellular communication. Yet, the analysis of the data generated by calcium imaging experiments demands a large amount of time from researchers. Tools enabling automated and semi-automated analysis are available, but often they allow automating only a part of the data analysis process. Therefore, we developed CALIMA (https://aethelraed.nl/calima), a free and open-source standalone software tool that provides an opportunity to quickly detect cells, to obtain the calcium spikes, and to determine the underlying network structure of neuronal cell cultures.

Methods

Owing to the difference of Gaussians algorithm applied for the cell detection, C

SUBMITTER: Radstake FDW 

PROVIDER: S-EPMC6718774 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature