Biological monitoring of o-toluidine in urine pretreated by an enzymatic deconjugation method.

ABSTRACT:

Objectives

To establish an enzymatic deconjugation method to separately quantify urinary o-toluidine (OT), its six metabolites, another six chemicals present in an OT-processing plant, and one metabolite of p-toluidine, and to propose optimal urinary biological monitoring items of OT exposure.

Methods

Thirty-six urine samples of an OT-processing plant's workers were obtained and pretreated by an enzymatic deconjugation method employing β-glucuronidase/arylsulfatase for 3 hours at 37°C and measured by liquid chromatograph-mass spectrometry (LC-MS). An alkaline hydrolytic pretreatment and 1-chlorobutane extraction procedure was also examined as a widely used urinary OT measurement method.

Results

The 14 chemicals were separated by LC-MS condition set by us and 13 chemic

SUBMITTER: Eitaki Y 

PROVIDER: S-EPMC6718832 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature