Integrated mRNA and miRNA profile expression in livers of Jinhua and Landrace pigs.

ABSTRACT:

Objective

To explore the molecular mechanisms of fat metabolism and deposition in pigs, an experiment was conducted to identify hepatic mRNAs and miRNAs expression and determine the potential interaction of them in two phenotypically extreme pig breeds.

Methods

mRNA and miRNA profiling of liver from 70-day Jinhua (JH) and Landrace (LD) pigs were performed using RNA sequencing. Blood samples were taken to detect results of serum biochemistry. Bioinformatics analysis were applied to construct differentially expressed miRNA-mRNA network.

Results

Serum total triiodothyronine (TT3) and total thyroxine (TT4) were significantly lower in Jinhua pigs, but the content of serum total cholesterol (TCH) and low-density lipoprotein cholesterol (LDLC) were strikingly higher. A total

SUBMITTER: Huang M 

PROVIDER: S-EPMC6718901 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature