Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite: Improvement of Loading Amount and Catalytic Activity.

ABSTRACT: In this study, a novel type of multi-armed polymer (poyltehylene glycol, PEG) magnetic graphene oxide (GO) composite (GO@Fe₃O₄@6arm-PEG-NH₂) has been synthesized as a support for immobilization of horseradish peroxidase (HRP) for the first time. The loading amount of HRP was relatively high (186.34 mg/g) due to the surface of carrier material containing a large amount of amino groups from 6arm-PEG-NH₂, but degradation rate of phenols was also much higher (95.4%), which is attributed to the synergistic effect between the free HRP (45.4%) and the support material of GO@Fe₃O₄@6arm-PEG-NH₂ (13.6%). Compared with the free enzyme, thermal, storage and operational stability of the immobilized HRP improved. The immobilize

SUBMITTER: Rong J 

PROVIDER: S-EPMC6718962 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature