Maintenance and enhancement of human peripheral blood mobilized stem/progenitor cell engraftment after ex vivo culture via an HDACi/SALL4 axis (3465).

ABSTRACT: Currently, there is a growing need for culturing hematopoietic stem/progenitor cells (HSPCs) in vitro for various clinical applications including gene therapy. Compared with cord blood (CB) CD34⁺ HSPCs, it is more challenging to maintain or expand CD34⁺ peripheral blood mobilized stem/progenitor cells (PBSCs) ex vivo. To fill this knowledge gap, we have systematically surveyed 466 small-molecule drug compounds for their potential in cytokine-dependent expansion of human CD34⁺CD90⁺ HSPCs. We found that epigenetic modifiers, especially histone deacetylase inhibitors (HDACis), could preferentially maintain and expand these cells. In particular, treatment of CD34⁺ PBSCs with a single dose of HDACi trichostatin A (TSA) at a concentration of

SUBMITTER: Tatetsu H 

PROVIDER: S-EPMC6719293 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature