The Preconditioning of Busulfan Promotes Efficiency of Human CD133+ Cells Engraftment in NOD Shi-SCID IL2Rγcnull (NOG) Mice via Intra-Bone Marrow Injection.

ABSTRACT: Human CD133⁺ stem cells were injected into the bone marrow cavity of NOG (NOD Shi-SCID IL2Rγc^null) mice with or without preconditioning of busulfan in order to assess the efficiency of human CD133⁺ cells engraftment. Peripheral blood from CD133⁺-engrafted NOG mice was analyzed by flow cytometry. The results showed that human CD19⁺ B lymphocytes could be detected at 4 weeks post-transplantation, and human CD4⁺, CD8⁺ subsets of T lymphocytes, CD19^- CD14^- HLA-DR⁺ DCs and CD19^- CD14⁺ monocytes could be detected at 16 weeks post-transplantation. The survival rate of mice in busulfan-untreated group (100%) was slightly higher than that in the busulfan-pretreated gro

SUBMITTER: Guo X 

PROVIDER: S-EPMC6719503 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature