Single-cell analysis of cardiogenesis reveals basis for organ-level developmental defects.

ABSTRACT: Organogenesis involves integration of diverse cell types; dysregulation of cell-type-specific gene networks results in birth defects, which affect 5% of live births. Congenital heart defects are the most common malformations, and result from disruption of discrete subsets of cardiac progenitor cells¹, but the transcriptional changes in individual progenitors that lead to organ-level defects remain unknown. Here we used single-cell RNA sequencing to interrogate early cardiac progenitor cells as they become specified during normal and abnormal cardiogenesis, revealing how dysregulation of specific cellular subpopulations has catastrophic consequences. A network-based computational method for single-cell RNA-sequencing analysis that predicts lineage-specifying transcription factors

SUBMITTER: de Soysa TY 

PROVIDER: S-EPMC6719697 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature