Integrated in vivo multiomics analysis identifies p21-activated kinase signaling as a driver of colitis.

ABSTRACT: Inflammatory bowel disease (IBD) is a chronic disorder of the gastrointestinal tract that has limited treatment options. To gain insight into the pathogenesis of chronic colonic inflammation (colitis), we performed a multiomics analysis that integrated RNA microarray, total protein mass spectrometry (MS), and phosphoprotein MS measurements from a mouse model of the disease. Because we collected all three types of data from individual samples, we tracked information flow from RNA to protein to phosphoprotein and identified signaling molecules that were coordinately or discordantly regulated and pathways that had complex regulation in vivo. For example, the genes encoding acute-phase proteins were expressed in the liver, but the proteins were detected by MS in the colon during inflammation.

SUBMITTER: Lyons J 

PROVIDER: S-EPMC6719711 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature