Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry.

ABSTRACT: Bacterial leaf scorch, caused by Xylella fastidiosa, is a major threat to blueberry production in the southeastern United States. Management of this devastating disease is challenging and often requires early detection of the pathogen to reduce major loss. There are several different molecular and serological detection methods available to identify the pathogen. Knowing the efficiency and suitability of these detection techniques for application in both field and laboratory conditions is important when selecting the appropriate detection tool. Here, we compared the efficiency and the functionality of four different molecular detection techniques (PCR, real-time PCR, LAMP and AmplifyRP® Acceler8™) and one serological detection technique (DAS-ELISA). The most sensitive method was found to be

SUBMITTER: Waliullah S 

PROVIDER: S-EPMC6719857 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature