Metaproteomics analysis of microbial diversity of human saliva and tongue dorsum in young healthy individuals.

ABSTRACT: Background: The human oral microbiome influences initiation or progression of diseases like caries or periodontitis. Metaproteomics approaches enable the simultaneous investigation of microbial and host proteins and their interactions to improve understanding of oral diseases. Objective: In this study, we provide a detailed metaproteomics perspective of the composition of salivary and tongue microbial communities of young healthy subjects. Design: Stimulated saliva and tongue samples were collected from 24 healthy volunteers, subjected to shotgun nLC-MS/MS and analyzed by the Trans-Proteomic Pipeline and the Prophane tool. Results: 3,969 bacterial and 1,857 human proteins could be identified from saliva and tongue, respectively. In total, 1,971 bacterial metapro

SUBMITTER: Rabe A 

PROVIDER: S-EPMC6720020 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature