An essential role of RNF187 in Notch1 mediated metastasis of hepatocellular carcinoma.

ABSTRACT:

Background

Aberrant activation of Notch signaling has been causally linked to the metastasis of hepatocellular carcinoma (HCC), however the underlying molecular mechanisms are still poorly understood. RING finger protein 187 (RNF187) was recently revealed to be a driver of several cancers, but its expression pattern and biological function in HCC are unknown.

Methods

The expression levels of Notch1 and RNF187 were assessed in two independent cohorts of HCC tissues, and modulation of Notch1 in HCC cells was performed to explore the regulatory role of Notch1 in HCC metastasis. RNA-sequencing (RNA-seq), bioinformatics analysis, luciferase reporter analysis, and chromatin immunoprecipitation assay (ChIP) were used to clarify the relationship between Notch1 signaling and its pote

SUBMITTER: Zhang L 

PROVIDER: S-EPMC6720101 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature