Adaptively evolved Escherichia coli for improved ability of formate utilization as a carbon source in sugar-free conditions.

ABSTRACT:

Background

Formate converted from CO₂ reduction has great potential as a sustainable feedstock for biological production of biofuels and biochemicals. Nevertheless, utilization of formate for growth and chemical production by microbial species is limited due to its toxicity or the lack of a metabolic pathway. Here, we constructed a formate assimilation pathway in Escherichia coli and applied adaptive laboratory evolution to improve formate utilization as a carbon source in sugar-free conditions.

Results

The genes related to the tetrahydrofolate and serine cycles from Methylobacterium extorquens AM1 were overexpressed for formate assimilation, which was proved by the ¹³C-labeling experiments. The amino acids detected by GC/MS showed signifi

SUBMITTER: Kim SJ 

PROVIDER: S-EPMC6720381 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature