Proteomic Analysis of Lipid Rafts from RBL-2H3 Mast Cells.

ABSTRACT: Lipid rafts are highly ordered membrane microdomains enriched in cholesterol, glycosphingolipids, and certain proteins. They are involved in the regulation of cellular processes in diverse cell types, including mast cells (MCs). The MC lipid raft protein composition was assessed using qualitative mass spectrometric characterization of the proteome from detergent-resistant membrane fractions from RBL-2H3 MCs. Using two different post-isolation treatment methods, a total of 949 lipid raft associated proteins were identified. The majority of these MC lipid raft proteins had already been described in the RaftProtV2 database and are among highest cited/experimentally validated lipid raft proteins. Additionally, more than half of the identified proteins had lipid modifications and/or transmembra

SUBMITTER: Freitas Filho EG 

PROVIDER: S-EPMC6720779 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature