Development of a quick dot blot assay for the titering of bovine ephemeral fever virus.

ABSTRACT:

Background

Bovine ephemeral fever virus (BEFV) causes fever and muscle stiffness in cattle, resulting in negative economic impact for cattle and dairy farms. During the manufacturing process of inactivated vaccine for virus control, it is important to determine the virus titer, but traditional methods such as plaque assay and TCID₅₀ assay require days of waiting time. We sought to develop a quick dot blot assay for BEFV titering.

Results

Three different kinds of BEFV antigens were prepared to raise primary antibodies for BEFV detection in dot blot assays: 1) purified BEFV particles, 2) Escherichia coli (E. coli)-expressed BEFV G1 region, and 3) E. coli-expressed BEFV N protein. Results showed that antibodies raised against purified BEFV particles can detect BEFV p

SUBMITTER: Cheng LT 

PROVIDER: S-EPMC6720828 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature