Ratiometric BRET Measurements of ATP with a Genetically-Encoded Luminescent Sensor.

ABSTRACT: Luciferase-based reporters provide a key measurement approach in a broad range of applications, from in vitro high-throughput screening to whole animal imaging. For example, luminescence intensity is widely used to measure promoter activity, protein expression levels, and cell growth. However, luminescence intensity measurements are subject to quantitative irregularities caused by luminescence decay and variation in reporter expression level. In contrast, bioluminescence resonance energy transfer (BRET) sensors provide the advantages of luciferase-based reporters but overcome the aforementioned irregularities because of the inherently ratiometric readout. Here, we generated a new ratiometric BRET sensor of ATP (ARSeNL-ATP detection with a Ratiometric mScarlet-NanoLuc sensor), and we demons

SUBMITTER: Min SH 

PROVIDER: S-EPMC6721044 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature