Crude Heparin Preparations Unveil the Presence of Structurally Diverse Oversulfated Contaminants.

ABSTRACT: Nowadays, pharmaceutical heparin is purified from porcine and bovine intestinal mucosa. In the past decade there has been an ongoing concern about the safety of heparin, since in 2008, adverse effects associated with the presence of an oversulfated chondroitin sulfate (OSCS) were observed in preparations of pharmaceutical porcine heparin, which led to the death of patients, causing a global public health crisis. However, it has not been clarified whether OSCS has been added to the purified heparin preparation, or whether it has already been introduced during the production of the raw heparin. Using a combination of different analytical methods, we investigate both crude and final heparin products and we are able to demonstrate that the sulfated contaminants are intentionally introduced in

SUBMITTER: Mendes A 

PROVIDER: S-EPMC6721129 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature