Epigenome Mapping Identifies Tumor-Specific Gene Expression in Primary Rectal Cancer.

ABSTRACT: Epigenetic alterations play a central role in cancer development and progression. The acetylation of histone 3 at lysine 27 (H3K27ac) specifically marks active genes. While chromatin immunoprecipitation (ChIP) followed by next-generation sequencing (ChIP-seq) analyses are commonly performed in cell lines, only limited data are available from primary tumors. We therefore examined whether cancer-specific alterations in H3K27ac occupancy can be identified in primary rectal cancer. Tissue samples from primary rectal cancer and matched mucosa were obtained. ChIP-seq for H3K27ac was performed and differentially occupied regions were identified. The expression of selected genes displaying differential occupancy between tumor and mucosa were examined in gene expression data from an independent pat

SUBMITTER: Flebbe H 

PROVIDER: S-EPMC6721540 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature