Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase.

ABSTRACT: The nickel-dependent carbon monoxide dehydrogenase (CODH) employs a unique heterometallic nickel-iron-sulfur cluster, termed the C-cluster, to catalyze the interconversion of CO and CO₂ Like other complex metalloenzymes, CODH requires dedicated assembly machinery to form the fully intact and functional C-cluster. In particular, nickel incorporation into the C-cluster depends on the maturation factor CooC; however, the mechanism of nickel insertion remains poorly understood. Here, we compare X-ray structures (1.50-2.48 Å resolution) of CODH from Desulfovibrio vulgaris (DvCODH) heterologously expressed in either the absence (DvCODH^-CooC) or presence (DvCODH^+CooC) of co-expressed CooC. We find that the C-cluster of DvCODH

SUBMITTER: Wittenborn EC 

PROVIDER: S-EPMC6721931 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature