Live-cell assays reveal selectivity and sensitivity of the multidrug response in budding yeast.

ABSTRACT: Pleiotropic drug resistance arises by the enhanced extrusion of bioactive molecules and is present in a wide range of organisms, ranging from fungi to human cells. A key feature of this adaptation is the sensitive detection of intracellular xenobiotics by transcriptional activators, activating expression of multiple drug exporters. Here, we investigated the selectivity and sensitivity of the budding yeast (Saccharomyces cerevisiae) multidrug response to better understand how differential drug recognition leads to specific activation of drug exporter genes and to drug resistance. Applying live-cell luciferase reporters, we demonstrate that the SNQ2, PDR5, PDR15, and YOR1 transporter genes respond to different mycotoxins, menadione, and hydrogen peroxide in

SUBMITTER: Vanacloig-Pedros E 

PROVIDER: S-EPMC6721934 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature