Intact-Mass Analysis Facilitating the Identification of Large Human Heart Proteoforms.

ABSTRACT: Proteoforms, the primary effectors of biological processes, are the different forms of proteins that arise from molecular processing events such as alternative splicing and post-translational modifications. Heart diseases exhibit changes in proteoform levels, motivating the development of a deeper understanding of the heart proteoform landscape. Our recently developed two-dimensional top-down proteomics platform coupling serial size exclusion chromatography (sSEC) to reversed-phase chromatography (RPC) expanded coverage of the human heart proteome and allowed observation of high-molecular weight proteoforms. However, most of these observed proteoforms were not identified due to the difficulty in obtaining quality tandem mass spectrometry (MS2) fragmentation data for large proteoforms from

SUBMITTER: Schaffer LV 

PROVIDER: S-EPMC6722015 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature