MHC-restricted Ag85B-specific CD8⁺ T cells are enhanced by recombinant BCG prime and DNA boost immunization in mice.

ABSTRACT: Despite efforts to develop effective treatments and vaccines, Mycobacterium tuberculosis (Mtb), particularly pulmonary Mtb, continues to provide major health challenges worldwide. To improve immunization against the persistent health challenge of Mtb infection, we have studied the CD8⁺ T cell response to Bacillus Calmette-Guérin (BCG) and recombinant BCG (rBCG) in mice. Here, we generated CD8⁺ T cells with an rBCG-based vaccine encoding the Ag85B protein of M. kansasii, termed rBCG-Mkan85B, followed by boosting with plasmid DNA expressing the Ag85B gene (DNA-Mkan85B). We identified two MHC-I (H2-K^d )-restricted epitopes that induce cross-reactive responses to Mtb and other related mycobacteria in both BALB/c (H2^d ) and CB6F1 (H2^b/d ) m

SUBMITTER: Komine-Aizawa S 

PROVIDER: S-EPMC6722017 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature