Generation of Human iPSC-Derived Intestinal Epithelial Cell Monolayers by CDX2 Transduction.

ABSTRACT:

Background & aims

To develop an effective and safe orally administered drug, it is important to predict its intestinal absorption rate, intestinal first-pass effect, and drug-drug interactions of orally administered drugs. However, there is no existing model to comprehensively predict the intestinal pharmacokinetics and drug-response of orally administered drugs. In this study, we attempted to generate homogenous and functional intestinal epithelial cells from human induced pluripotent stem (iPS) cells for pharmaceutical research.

Methods

We generated almost-homogenous Villin- and zonula occludens-1 (ZO1)-positive intestinal epithelial cells by caudal-related homeobox transcription factor 2 (CDX2) transduction into human iPS cell-derived intestinal progenitor cells.

Resul

SUBMITTER: Takayama K 

PROVIDER: S-EPMC6722387 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature