Effect of Environment on the Evolutionary Trajectories and Growth Characteristics of Antibiotic-Resistant <i>Escherichia coli</i> Mutants.

ABSTRACT: The fitness cost to bacteria of acquisition of resistance determinants is critically under-investigated, and the identification and exploitation of these fitness costs may lead to novel therapeutic strategies that prevent the emergence of antimicrobial resistance. Here we used Escherichia coli and amoxicillin-clavulanic acid (AMC) resistance as a model to understand how the artificial environments utilized in studies of bacterial fitness could affect the emergence of resistance and associated fitness costs. Further, we explored the predictive value of this data when strains were grown in the more physiologically relevant environments of urine and urothelial organoids. Resistant E. coli isolates were selected for following 24-h exposure to sub-inhibitory concentrations of AMC

SUBMITTER: Hubbard ATM 

PROVIDER: S-EPMC6722461 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature