Multidimensional profiling of drug-treated cells by Imaging Mass Cytometry.

ABSTRACT: In pharmaceutical research, high-content screening is an integral part of lead candidate development. Measuring drug response in vitro by examining over 40 parameters, including biomarkers, signaling molecules, cell morphological changes, proliferation indices, and toxicity in a single sample, could significantly enhance discovery of new therapeutics. As a proof of concept, we present here a workflow for multidimensional Imaging Mass Cytometry™ (IMC™) and data processing with open source computational tools. CellProfiler was used to identify single cells through establishing cellular boundaries, followed by histoCAT™ (histology topography cytometry analysis toolbox) for extracting single-cell quantitative information visualized as t-SNE plots and heatmaps. Human breast cancer-derived cell

SUBMITTER: Bouzekri A 

PROVIDER: S-EPMC6722888 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature