Newly identified APN splice isoforms suggest novel splicing mechanisms may underlie circRNA circularization in moth.

ABSTRACT: Circular RNA (circRNA) have long been considered by-products of splicing errors, but the coordination of RNA transcription and exon circularization events remains poorly understood. Here, we investigated this question using genes encoding aminopeptidases N (APNs), which are receptors of Bacillus thuringiensis toxins, in the cotton bollworm, Helicoverpa armigera. We cloned and sequenced the cDNA of ten APN genes (HaAPN1-10) located in the same APN gene cluster, and detected 20 and 14 novel splicing isoforms with exon skipping in HaAPN1 and HaAPN3, respectively, whereas no or very few variants were found in the remaining genes. Further study identified 14 and 6 circular RNA (circRNA) in HaAPN1 and HaAPN3, respectively. Neither novel splicing isoforms nor circRNA were detected in HaAPN2 and H

SUBMITTER: Gao M 

PROVIDER: S-EPMC6722899 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature