Genetic Mapping of Prince Rupprecht's Larch (Larix principis-rupprechtii Mayr) by Specific-Locus Amplified Fragment Sequencing.

ABSTRACT: A high-density genetic linkage map is essential for plant genetics and genomics research. However, due to the deficiency of genomic data and high-quality molecular markers, no genetic map has been published for Prince Rupprecht's larch (Larix principis-rupprechtii Mayr), a conifer species with high ecological and commercial value in northern China. In this study, 145 F1 progeny individuals from an intraspecific cross between two elite clones of L. principis-rupprechtii and their parents were employed to construct the first genetic map in this important tree species using specific-locus amplified fragment sequencing (SLAF-seq). After preprocessing, the procedure yielded 300.20 Gb of raw data containing 1501.22 M pair-end reads. A total of 324,352 SNP markers were detect

SUBMITTER: Dong M 

PROVIDER: S-EPMC6723236 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature