Identification and manipulation of Neurospora crassa genes involved in sensitivity to furfural.

ABSTRACT:

Background

Biofuels derived from lignocellulosic biomass are a viable alternative to fossil fuels required for transportation. Following plant biomass pretreatment, the furan derivative furfural is present at concentrations which are inhibitory to yeasts. Detoxification of furfural is thus important for efficient fermentation. Here, we searched for new genetic attributes in the fungus Neurospora crassa that may be linked to furfural tolerance. The fact that furfural is involved in the natural process of sexual spore germination of N. crassa and that this fungus is highly amenable to genetic manipulations makes it a rational candidate for this study.

Results

Both hypothesis-based and unbiased (random promotor mutagenesis) approaches were performed to identify

SUBMITTER: Feldman D 

PROVIDER: S-EPMC6724289 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature