TEX9 and eIF3b functionally synergize to promote the progression of esophageal squamous cell carcinoma.

ABSTRACT: BACKGROUND:Esophageal squamous cell carcinoma (ESCC) is one of the most frequent malignant digestive tumors around the world. We previously demonstrated that eIF3b could promote the progression of ESCC. The exact mechanisms underlying these effects remained unknown. METHODS:Quantitative proteomics was applied to detect the potential targets of Eukaryotic translation initiation factor 3 subunit b (eIF3b). RT-qPCR and Western blot were performed to detect the expression of targeted gene and pathway related genes. RNA-immunoprecipitation was applied to verify the binding of eIF3b with targeted gene. Moreover, CCK-8 assay, colony-formation assay, transwell assay, flow cytometry for cell apoptosis and tumor xenograft assay were performed to analyze the regulation of the targeted gene on the bio

SUBMITTER: Xu F 

PROVIDER: S-EPMC6724304 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature