Electronegative LDL from Rabbits Fed with Atherogenic Diet Is Highly Proinflammatory.

ABSTRACT: Electronegative low-density lipoprotein (LDL(-)) has been found in the plasma of familial hypercholesterolemia and acute myocardial infarction and has been implicated in atherosclerosis and cardiovascular disease. However, less is known about the involvement of LDL(-) in atherosclerosis-related inflammation. This study aims at investigating the inducibility of LDL(-) by atherogenic diet in rabbits and at exploring the proinflammatory potential of the diet-induced LDL(-) in macrophages. Rabbits were fed with an atherogenic diet; LDL was isolated from plasma by NaBr density gradient ultracentrifugation and was then resolved into nLDL and LDL(-) by anion-exchange chromatography. Isolated nLDL and LDL(-) were directly used or incubated with 10 μM CuSO₄ for 24 h to produce cop

SUBMITTER: Chang PY 

PROVIDER: S-EPMC6724430 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature