Yb body assembly on the flamenco piRNA precursor transcripts reduces genic piRNA production.

ABSTRACT: In Drosophila ovarian somatic cells, PIWI-interacting small RNAs (piRNAs) against transposable elements are mainly produced from the ∼180-kb flamenco (flam) locus. flam transcripts are gathered into foci, located close to the nuclear envelope, and processed into piRNAs in the cytoplasmic Yb bodies. The mechanism of Yb body formation remains unknown. Using RNA fluorescence in situ hybridization, we found that in the follicle cells of ovaries the 5'-ends of flam transcripts are usually located in close proximity to the nuclear envelope and outside of Yb bodies, whereas their extended downstream regions mostly overlap with Yb bodies. In flam^KG mutant ovaries, flam transcripts containing the first and, partially, second exons but lack

SUBMITTER: Sokolova OA 

PROVIDER: S-EPMC6724695 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature