A circadian clock and light/dark adaptation differentially regulate adenosine in the mammalian retina.

ABSTRACT: Although the purine adenosine acts as an extracellular neuromodulator in the mammalian CNS in both normal and pathological conditions and regulates sleep, the regulation of extracellular adenosine in the day and night is incompletely understood. To determine how extracellular adenosine is regulated, rabbit neural retinas were maintained by superfusion at different times of the regular light/dark and circadian cycles. The adenosine level in the superfusate, representing adenosine overflow from the retinas, and the adenosine level in retinal homogenates, representing adenosine content, were measured using HPLC with fluorescence detection in the absence or presence of blockers of adenosine transport and/or extracellular adenosine synthesis. We report that darkness, compared with illumination,

SUBMITTER: Ribelayga C 

PROVIDER: S-EPMC6725211 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature